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KMID : 1034320170080010026
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Sleep Medicine Research
2017 Volume.8 No. 1 p.26 ~ p.32
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Sleep Fragmentation Induces Activation of NOD-Like Receptor Protein-3 Inflammasome in Rat Hippocampus
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Yoon Dae-Wui
Shin Sung-Jin
Choi Jin-Ny
Shin Claire
Lee Chris
Lee Jen-Na
Hong Huck-Jun
Jo Richard
Lee Tommy
Kim Jeff
Jun Nu-Ri
Lee Seung-Ku
Kim Jin-Kwan
Jwa Hoon-Jung
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Abstract
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Background and Objective: Continuous sleep is important for cognitive function; studies have shown that disrupted sleep has a deleterious effect on hippocampus-dependent cognitive function, but the exact mechanism is unknown. The inflammasome NOD-like receptor protein-3 (NLRP-3) has been reported to cause memory and cognitive dysfunction, but no studies have examined whether NLRP-3 is up-regulated in the hippocampus by sleep fragmentation (SF). Therefore, we investigated whether SF affected NLRP-3 activation in the rat hippocampus in a wheel-based SF model.
Methods: We randomly divided 7-week-old male Wistar rats into 4-day SF, 4-day exercise control (EC), 8-day SF, and 8-day EC groups. SF was accomplished with a forced walking wheel with a 30-s on/90-s off cycle, and we set EC at 10 min on/30 min off. We performed Western blots to compare NLRP-3 expression levels, and we measured malondialdehyde (MDA) levels in the hippocampus with a commercial kit.
Results: The SF group had more but shorter NREM bouts. Western blot analysis revealed that 4 and 8 days of SF up-regulated the expression of the NLRP-3 complex in the hippocampus including ASC, caspase-1, and IL-1β. Thioredoxin-interacting protein expression also increased more in the SF group, and the MDA level increases were greater in the SF groups than in the corresponding EC groups.
Conclusions: SF up-regulates NLRP-3 in the hippocampus. Additional behavioral and mechanistic studies are required to clarify the role of NLRP-3 in the hippocampi of rats subjected to SF.
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KEYWORD
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Sleep fragmentation, NLRP-3, Hippocampus, Malondialdehyde
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